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In Vitro Fertilization (IVF)

What is IVF?

The term “ in vitro fertilization” refers to medically assisted reproduction. The process of fertilization between the two gametes, the oocyte and the spermatozoon, takes place outside the woman’s body in the laboratory.

This method involves a wide array of techniques, which aim at helping infertile couples to have children.

Appropriate counseling by an IVF expert plays a paramount role in helping the couple decide whether to proceed with an assisted reproduction treatment. The couple must know if this type of therapy is necessary or if a milder treatment can achieve the aim of reproduction.

IVF is divided in four basic phases:


  • Induction of multiple follicular development
  • Oocyte retrieval
  • Fertilization-embryo culture
  • Embryo transfer


During ovarian stimulation (induction of follicular development) specific  treatment protocols are followed: medication is administered to the patient, who must undergo regular (every 2-3 days) sonographic and hormonal monitoring.

When the first phase of ovarian stimulation has been completed, we proceed to oocyte retrieval, whereby the oocytes are retrieved from the woman in the IVF unit under general anaesthesia. On the same day oocyte insemination occurs in the laboratory using the husband’s spermatozoa.

The embryos which emerge through this procedure are developed under specific conditions (the use of special materials for embryo culture) in incubators, where their environment is formed based on modern-day studies on low concentration oxygen.

Embryo transfer can occur on days 2, 3 or 5-6 of embryo development. After a detailed evaluation of embryo quality by a clinical embryologist, the highest- quality embryos are selected, which will then be transferred into the maternal uterus



New IVF Techniques

Intracytoplasmic sperm injection – ICSI)

The technique of intracytoplasmic sperm injection (ICSI) is now used in the majority of cases of IVF treatment, since it achieves higher rates of oocyte fertilization.

With  the ICSI technique, a single, healthy sperm is injected with a special needle into the cytoplasm of the oocyte to fertilise it.


Time-Lapse Embryo Monitoring


In recent years, the time-lapse technique has altered the way of evaluating embryos during their incubation before embryo transfer.

The technique is based on the constant monitoring of embryos in the incubator, in which a special camera has been placed. This camera provides invaluable information concerning the embryos’ morphological development by constant time-lapse imaging (it takes pictures every 5 minutes). These images are stored to be evaluated at a later time, prior to the selection of embryos for embryo-transfer.

A basic advantage of this technique is that the embryos are not removed from the protected environment in the incubator in order to be evaluated by the embryologist.

Furthermore, this technique provides the expert with more evaluation criteria for monitoring, which may not be visible during the screenings performed at specified times outside the incubator.

By providing more detailed information on embryological dynamics, the continuous, non-invasive character of the time-lapse technique allows us to select the embryos with the greatest implantation potential and reduce the number of embryos which are transferred into the uterus. Thus, multiple birth rates are reduced.

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